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共聚焦多色成像在癌症研究和免疫学中的潜力
在本次网络研讨会上,来自莫纳什制药科学研究所的CameronNowell和他的同事将分享他们在多重成像方面的经验,以及他们通过巧妙的共聚焦成像采集和利用FLIM等其他多重成像模式所取得的成果。
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可重复性、协作和新成像技术的力量
在本次网络研讨会上,您将了解到影响显微镜可重复性的因素,有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
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How FLIM Microscopy Helps to Detect Microplastic Pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…
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Development of Fluorescence Lifetime Imaging Microscopy (FLIM) and its Relevance for Functional Imaging
Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager functional imaging, during Leica Microsystems Meets…
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FRAP实验步骤式指南
漂白后荧光恢复(FRAP)已被认定为观察大分子平移扩散过程方面使用最为广泛的一种方法。由此产生的信息可用于确定动力学性质,如扩散系数、流动分数和荧光标记分子的传输速率。FRAP实验利用了短激光脉冲的荧光团辐照。先进的激光扫描显微镜如TCS…
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Widefield Calcium Imaging with Calcium Indicator Fura2
In eukaryotic cells Ca2+ is one of the most widespread second messengers used in signal transduction pathways. Intracellular levels of Ca2+ are usually kept low, as Ca2+ often forms insoluble…
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Fluorescence Correlation Spectroscopy (FCS)
Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…
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Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…