12
–
13
Nov
2024
类器官与器官芯片技术网络研讨会-仪器信息网
China
•
Webinar
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![[Translate to chinese:] Multicolor fixed STED image. Inner ear section, mouse, TauSTED Xtend 589 on AF488 and TauSTED Xtend 775 on AF633-Phalloidin. Sample courtesy of Dennis Derstrof, Klinik für Hals-, Nasen und Ohrenheilkunde, Universität Marburg & Prof. Dr. Dominik Oliver aus dem Institut für Physiologie und Pathophysiologie, Abteilung für Neurophysiologie, Universität Marburg.](/fileadmin/_processed_/c/a/csm_Inner_ear_section_mouse_multicolor_fixed_TauSTED_Xtend_1a1a0b424b.jpg "[Translate to chinese:] Multicolor fixed STED image. Inner ear section, mouse, TauSTED Xtend 589 on AF488 and TauSTED Xtend 775 on AF633-Phalloidin.")
细胞活成像的纳米级扩展
新的STED显微技术方法——TauSTED Xtend，使得在纳米级别下对活体完整样本进行扩展多色成像成为可能。通过结合空间和寿命信息，TauSTED Xtend提供了额外一层信息，允许在极低的光剂量下分辨小细节并在整体结构中解析它们。
![[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD](/fileadmin/_processed_/6/3/csm_Mouse_pial_and_cortical_vasculature_f409d941d0.jpg "[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD")
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![[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.](/fileadmin/_processed_/8/7/csm_Lifetime-based_multiplexing_in_live_cells_using_TauSeparation_8fa372a6b9.jpg "[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green.")
可重复性、协作和新成像技术的力量
在本次网络研讨会上，您将了解到影响显微镜可重复性的因素，有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
![[Translate to chinese:] Combining spectrally resolved detection and fluorescence lifetime multiplexing](/fileadmin/academy/2022/Abstracts_Interviews_Webinars/Combining_spectrally_resolved_detection_and_fluorescence_lifetime_multiplexing.jpg "[Translate to chinese:] Combining spectrally resolved detection and fluorescence lifetime multiplexing")
