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![[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.](/fileadmin/_processed_/8/7/csm_Lifetime-based_multiplexing_in_live_cells_using_TauSeparation_c5d58adf13.jpg "[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green.")
可重复性、协作和新成像技术的力量
在本次网络研讨会上，您将了解到影响显微镜可重复性的因素，有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
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![[Translate to chinese:] Advanced technologies support neurosurgical teaching. Image courtesy of Dr. Florian Bernard.](/fileadmin/_processed_/b/0/csm_Advanced_technologies_support_neurosurgical_teaching_6e7281859a.jpg "[Translate to chinese:] Advanced technologies support neurosurgical teaching. Image courtesy of Dr. Florian Bernard.")
3D、AR和VR技术在神经外科教学领域的应用
尽管拥有强大的传统教学方法，但神经外科解剖学依然是一门艰难晦涩的课程。因为这些传统教学方法并不能确保良好的知识传授效果，尤其是经验丰富的外科医生的提示和技巧。模拟解剖和现场教学等新的方法有助于提高学习质量，同时满足数字化程度更高的新一代实习医生的需求。
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![[Translate to chinese:] THY1-EGFP labeled neurons in mouse brain processed using the PEGASOS 2 tissue clearing method, imaged on a Leica confocal microscope. Neurons were traced using Aivia’s 3D Neuron Analysis – FL recipe. Image credit: Hu Zhao, Chinese Institute for Brain Research.](/fileadmin/_processed_/f/1/csm_Neurons_in_mouse_brain_with_Aivia_3D_Neuron_Analysis_94d3981e50.jpg "[Translate to chinese:] THY1-EGFP labeled neurons in mouse brain processed using the PEGASOS 2 tissue clearing method. Neurons were traced using Aivia’s 3D Neuron Analysis – FL recipe. Image credit: Hu Zhao, Chinese Institute for Brain Research.")
借助人工智能，揭示复杂而密集的神经元图像中的洞察
神经元的3D形态学分析通常需要使用不同的成像模式，捕捉多种类型的神经元，并在各种密度下相连的传统Leica SP8显微镜采集多达解神经元的形态，这对许多研究人员来说仍然是一个耗时的挑战。
![[Translate to chinese:] Cancer cells](/fileadmin/_processed_/6/b/csm_Cancer_cells_f5e991d1c9.jpg "[Translate to chinese:] Cancer cells")
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![[Translate to chinese:] Use of GLOW800 Augmented Reality during AVM surgery. Image courtesy of Prof. Philippe Bijlenga.](/fileadmin/_processed_/f/9/csm_Use_of_GLOW800_Augmented_Reality_during_AVM_surgery_43b0e20a9b.jpg "[Translate to chinese:] Use of GLOW800 Augmented Reality during AVM surgery. Image courtesy of Prof. Philippe Bijlenga.")
动脉瘤夹闭和AVM手术中的现实荧光
多数神经血管手术是脑外科手术。保护大脑非常重要。脑血管也至关重要，并且闭塞时间有限。在徕卡神经可视化峰会上，Philippe Bijlenga教授分享了他使用GLOW800增强现实荧光技术执行动脉瘤夹闭术和AVM手术的经验以及此方法的优势。
