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不同组织样品制备方法的RNA质量
本文介绍了样品制备过程和紫外激光显微切片(UV LMD)对小鼠脑组织冷冻切片RNA质量的影响。为在提取RNA时获取良好的结果,从高品质组织开始并在处理前后检查RNA质量至关重要。RNA完整性指数(RIN)以1到10的等级标准来显示样品质量。简言之,RIN数值越高,RNA质量越高。这项研究结果表明,可以利用紫外激光显微切片技术,可以从单个组织细胞中获取品质稳定的RNA。
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Live Cell Isolation by Laser Microdissection
Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections,…
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Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own…
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Preparation of Laser Microdissected Plant Cell and Tissue Material for Microchemical Analysis
Secondary plant metabolites are involved in various plant physiological and biochemical processes, allowing plants to interact successfully with their biotic and abiotic environment. Distribution…
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Video: How to Achieve Fast Precise Cutting Lines in Laser Microdissection
This video explains the laser beam deflection principle of Leica Microsystems‘ laser microdissection systems (Leica LMD). The laser, the beam expander, motorized attenuator, motorized iris diaphragm…
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An Introduction to Laser Microdissection
Laser microdissection is a highly selective process for preparing samples for DNA, RNA and protein analysis. It is a microscope-controlled manipulation technique for the precise separation of samples…
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Applications of Laser Microdissection
Laser microdissection and laser micromanipulation are suitable for gaining a differentiated insight into the function of genes and proteins, and are used for a wide range of applications in…