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Video: The White Light Laser – How to Effectively Excite Multiple Fluorophores with a Single Light Source
The Leica White Light Laser produces a continuous spectral output between the wavelengths of 470 and 670 nm. It allows you to select 8 excitation lines from 3 trillion unique combinations for…
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Joseph Gall的视频讲座:显微镜的早期历史
Joseph Gall带我们了解早期显微镜的历史和细胞的发现。复式显微镜是在17世纪与望远镜一起发明的;然而,由于光学畸变,这些显微镜直到19世纪末才被广泛使用。在此期间,简单的显微镜在整个18世纪和19世纪被用于生物学的重大发现,包括对细胞核、纤毛、细胞、细菌和原生动物的首次描述。光学技术在19世纪中后期得到改善厚,复合显微镜就被用来发现染色体、有丝分裂和其他细胞结构。
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Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis
Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or…
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![[Translate to chinese:] Watch imaged with DMS300. [Translate to chinese:] Watch imaged with DMS300.](/fileadmin/_processed_/4/8/csm_Watch_with_DMS300_00fad3276b.jpg)
关于数码显微镜,您一直想知道但从未问过的问题
数码显微镜是显微镜领域的热门词汇之一,其中有几个事实值得了解。徕卡显微系统公司的产品经理 Georg Schlaffer 经常被客户和同事问及有关数码显微系统的问题。他与科学撰稿人 Jim DeRose 合作,为最重要的问题提供了全面的答案。
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![Schematic graph of the light path in a Spalt-Ultramikroskop. Schematic graph of the light path in a Spalt-Ultramikroskop.](/fileadmin/_processed_/b/7/csm_Light_path_in_a_Spalt-Ultramikroskop_e00478181a.jpg)
Confocal and Light Sheet Imaging
Optical imaging instrumentation can magnify tiny objects, zoom in on distant stars and reveal details that are invisible to the naked eye. But it notoriously suffers from an annoying problem: the…
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![Leica LMD7 Leica LMD7](/fileadmin/_processed_/6/c/csm_LMD-Consumables_d041cba518.jpg)
Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own…
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Universal PAINT – Dynamic Super-Resolution Microscopy
Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…
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冷冻透射电子显微镜的投入式冷冻技术:应用
低温下观察完全含水、未染色样本的透射电子显微镜(cryo TEM)是结构生物学、细胞生物学、药理学和其他科学分支的通用工具。通过将标本放入冷冻剂中进行超快速冷冻(投入式冻结)是一种常用的方法,用于制备在透射电镜观察的各种标本。本文是对投入式冷冻的补充,介绍了在不同领域使用投入式冷冻标本的三种冷冻TEM应用。
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冷冻透射电子显微镜的投入式冷冻技术:基本原理
透射电子显微镜(TEM)所需的高真空度极大地减弱了研究自然存在于水相中的标本的能力:将“湿”标本暴露在远低于水蒸汽压的压力下,将导致水相在真空中迅速沸腾,对标本的结构造成破坏性后果。因此,在常规TEM中使用了各种方法在显微观察前干燥标本——这样一个样品制备步骤,通常导致实验结果出现假象。