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![[Translate to chinese:] Multicolor fixed STED image. Inner ear section, mouse, TauSTED Xtend 589 on AF488 and TauSTED Xtend 775 on AF633-Phalloidin. Sample courtesy of Dennis Derstrof, Klinik für Hals-, Nasen und Ohrenheilkunde, Universität Marburg & Prof. Dr. Dominik Oliver aus dem Institut für Physiologie und Pathophysiologie, Abteilung für Neurophysiologie, Universität Marburg.](/fileadmin/_processed_/c/a/csm_Inner_ear_section_mouse_multicolor_fixed_TauSTED_Xtend_09ebb898fd.jpg "[Translate to chinese:] Multicolor fixed STED image. Inner ear section, mouse, TauSTED Xtend 589 on AF488 and TauSTED Xtend 775 on AF633-Phalloidin.")
细胞活成像的纳米级扩展
新的STED显微技术方法——TauSTED Xtend，使得在纳米级别下对活体完整样本进行扩展多色成像成为可能。通过结合空间和寿命信息，TauSTED Xtend提供了额外一层信息，允许在极低的光剂量下分辨小细节并在整体结构中解析它们。
![[Translate to chinese:] Spheroid stained with Cyan: Dapi nuclear countertain; Green AF488 Involucrin; Orange AF55 Phalloidin Actin; Magenta AF647 CK14.](/fileadmin/_processed_/6/8/csm_Spheroid_stained_with_Cyan_4color_overlay_78dff87b83.jpg "[Translate to chinese:] Spheroid stained with Cyan: Dapi nuclear countertain; Green AF488 Involucrin; Orange AF55 Phalloidin Actin; Magenta AF647 CK14.")
![[Translate to chinese:] Five-color FLIM-STED](/fileadmin/_processed_/a/3/csm_5-color_FLIM-STED_a783161004.jpg "[Translate to chinese:] Five-color FLIM-STED")
![[Translate to chinese:] Combining spectrally resolved detection and fluorescence lifetime multiplexing](/fileadmin/_processed_/6/3/csm_Combining_spectrally_resolved_detection_and_fluorescence_lifetime_multiplexing_1b411eea2d.jpg "[Translate to chinese:] Combining spectrally resolved detection and fluorescence lifetime multiplexing")
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通过非拟合且简便的 FRET-FLIM 方法可视化蛋白质 - 蛋白质相互作用
了解活细胞中的分子相互作用对于解读大多数细胞功能背后的分子机制至关重要。研究蛋白质-蛋白质相互作用的金标准是福斯特共振能量转移（FRET）。尽管有几种方法可以在生物样品中证明FRET，但使用荧光寿命成像显微镜（FLIM）可以基于仅供体荧光的行为直接量化FRET。
