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先进的细胞超微结构研究
冷冻断裂和冷冻蚀刻是研究柔性膜相关结构(如紧密连接或肠道糖萼)的有用工具。冷冻断裂和冷冻蚀刻是两种互补的方法,通过样品玻璃化来保护目标结构,然后断开冷冻标本以揭示内部结构。冷冻蚀刻是一个后续步骤,在真空下表面冰升华以揭示更多细节。在这些技术中,喷镀金属或碳使样品能够直接在冷冻扫描电子显微镜(SEM)中成像,或作为复制膜在透射电子显微镜(TEM)中成像。这对技术用于研究细胞器、膜、层和乳液,特别适用…
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Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…
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High-pressure freezing: Revealing functional mechanisms of synaptic transmission
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…
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Expanding the Limits of Electron Microscopy Sample Preparation
Capturing the intricate changes in fine structure or in cell dynamics with conventional cryo solutions can be challenging sometimes. Leica Microsystems has developed a new cryo platform, the Leica EM…
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非聚焦离子束研磨的实践应用
机械抛光不仅费时费力,而且还会产生伪影,干扰扫描电子显微镜(SEM)的电子背散射衍射(EBSD)结果或光学显微镜研究。相比之下,离子束研磨可以消除干扰数据分析和图像解读的伪影。