09
Apr
2025
CYTO U Live Web Event
United States
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Webinar
10
Apr
2025
中国细胞生物学学会线上直播
China
•
Webinar
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![[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD](/fileadmin/_processed_/6/3/csm_Mouse_pial_and_cortical_vasculature_d3e7a9948c.jpg "[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD")
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![[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.](/fileadmin/_processed_/8/7/csm_Lifetime-based_multiplexing_in_live_cells_using_TauSeparation_c5d58adf13.jpg "[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green.")
可重复性、协作和新成像技术的力量
在本次网络研讨会上，您将了解到影响显微镜可重复性的因素，有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
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![[Translate to chinese:] THY1-EGFP labeled neurons in mouse brain processed using the PEGASOS 2 tissue clearing method, imaged on a Leica confocal microscope. Neurons were traced using Aivia’s 3D Neuron Analysis – FL recipe. Image credit: Hu Zhao, Chinese Institute for Brain Research.](/fileadmin/_processed_/f/1/csm_Neurons_in_mouse_brain_with_Aivia_3D_Neuron_Analysis_94d3981e50.jpg "[Translate to chinese:] THY1-EGFP labeled neurons in mouse brain processed using the PEGASOS 2 tissue clearing method. Neurons were traced using Aivia’s 3D Neuron Analysis – FL recipe. Image credit: Hu Zhao, Chinese Institute for Brain Research.")
借助人工智能，揭示复杂而密集的神经元图像中的洞察
神经元的3D形态学分析通常需要使用不同的成像模式，捕捉多种类型的神经元，并在各种密度下相连的传统Leica SP8显微镜采集多达解神经元的形态，这对许多研究人员来说仍然是一个耗时的挑战。
![[Translate to chinese:] Cancer cells](/fileadmin/_processed_/6/b/csm_Cancer_cells_f5e991d1c9.jpg "[Translate to chinese:] Cancer cells")
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![[Translate to chinese:] Masson-Goldner staining of a hedgehog brain slice.](/fileadmin/_processed_/c/0/csm_Masson-Goldner_staining_of_hedgehog_brain_slice_a392b39de3.jpg "[Translate to chinese:] Masson-Goldner staining of a hedgehog brain slice.")
如何利用单个系统对组织学荧光样品进行成像
在本集MicaCame中，主持人Lynne Turnbull和Patric Pelzer将带您探寻生物样本染色的历史之旅。他们将解释为什么您通常必须选择为组织学样品或荧光样品选择特定的系统，以及如何利用新的成像技术克服这一点。
