09
Apr
2025
CYTO U Live Web Event
United States
•
Webinar
10
Apr
2025
中国细胞生物学学会线上直播
China
•
Webinar
Filter articles
标签
产品
![[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD](/fileadmin/_processed_/6/3/csm_Mouse_pial_and_cortical_vasculature_d3e7a9948c.jpg "[Translate to chinese:] In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD")
Loading...
![[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.](/fileadmin/_processed_/8/7/csm_Lifetime-based_multiplexing_in_live_cells_using_TauSeparation_c5d58adf13.jpg "[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green.")
可重复性、协作和新成像技术的力量
在本次网络研讨会上，您将了解到影响显微镜可重复性的因素，有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
![[Translate to chinese:] Combining spectrally resolved detection and fluorescence lifetime multiplexing](/fileadmin/_processed_/6/3/csm_Combining_spectrally_resolved_detection_and_fluorescence_lifetime_multiplexing_1b411eea2d.jpg "[Translate to chinese:] Combining spectrally resolved detection and fluorescence lifetime multiplexing")
Loading...
通过非拟合且简便的 FRET-FLIM 方法可视化蛋白质 - 蛋白质相互作用
了解活细胞中的分子相互作用对于解读大多数细胞功能背后的分子机制至关重要。研究蛋白质-蛋白质相互作用的金标准是福斯特共振能量转移（FRET）。尽管有几种方法可以在生物样品中证明FRET，但使用荧光寿命成像显微镜（FLIM）可以基于仅供体荧光的行为直接量化FRET。
