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Overcoming Challenges with Microscopy when Imaging Moving Zebrafish Larvae
Zebrafish is a valuable model organism with many beneficial traits. However, imaging a full organism poses challenges as it is not stationary. Here, this case study shows how zebrafish larvae can be…
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Aneurysm Clipping: Assessing Perforators in Real-Time with AR Fluorescence
This article covers two aneurysm clipping cases highlighting the clinical benefits of GLOW800 Augmented Reality Fluorescence application in neurosurgery, based on insights from Prof. Tohru Mizutani,…
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Advancing Uterine Regenerative Therapies with Endometrial Organoids
Prof. Kang's group investigates important factors that determine the uterine microenvironment in which embryo insertion and pregnancy are successfully maintained. They are working to develop new…
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![[Translate to chinese:] Optical microscope image, which is a composition of both brightfield and fluorescence illumination, showing organic contamination on a wafer surface. [Translate to chinese:] Optical microscope image, which is a composition of both brightfield and fluorescence illumination, showing organic contamination on a wafer surface. The inset images in the upper left corner show the brightfield image (above) and fluorescence image (below with dark background).](/fileadmin/_processed_/a/1/csm_Organic_contamination_on_a_wafer_surface_6699165cee.jpg)
晶圆上的光刻胶残留和有机污染物的可视化
随着半导体上集成电路(IC)的尺寸低于10纳米,在晶圆检测中有效检测光刻胶残留等有机污染物和缺陷变得越来越重要。光学显微镜仍然是常见的检测方法,但对于有机污染物,明场和其他类型的照明可能会存在局限性。本文讨论了荧光显微镜如何在半导体行业的QC、故障分析和研发过程中有效检测晶圆上的光刻胶残留和其他有机污染物。
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激光显微切割技术用于组织和细胞分离的协议 - 免费下载电子书
激光显微切割(LMD,也称为激光捕获显微切割或LCM)使用户能够分离特定的单个细胞或整个组织区域,甚至亚细胞结构如染色体。纯化的组织和细胞可用于下游的RNA、DNA和蛋白质组工作流程。
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数码显微镜摄像头和图像分析的基础技术术语定义
现今绝大多数显微镜都配置了摄像头。摄像头的特征通常决定了所采集到的图像是否能够揭示出研究人员希望观察到的现象。但深入到摄像头术语时,技术术语十分繁杂。我们汇总整理了最为重要的术语及其简明释意以便提供方向。这些术语按字母顺序排列。
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![[Translate to chinese:] Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light. [Translate to chinese:] Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.](/fileadmin/_processed_/0/6/csm_Fluorescence_microscopy_image_of_liver_tissue_0748f2a4d5.jpg)
落射荧光显微镜和反射对比显微镜
多年来,荧光显微镜一直仅使用透射光和暗场照明。随着时间的推移,对改进照明的需求不断增长,这导致了落射照明(也称为入射光照明)的发展。经过 40 年的发展和改进,落射照明荧光显微镜已成为生命科学、临床医学诊断和材料科学领域常规实验室工作和研究的实用方法。大部分开发工作由 Ploem 集团和 Leitz 公司(现为 Leica Microsystems)完成。
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![[Translate to chinese:] C. elegans adult hermaphrodite gonades acquired using THUNDER Imager. Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany. [Translate to chinese:] C. elegans adult hermaphrodite gonades acquired using THUNDER Imager. Staining: blue - DAPI (nucleus), green - SP56 (sperm), red - RME-2 (oocyte), magenta - PGL-1 (RNA + protein granules). Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany.](/fileadmin/_processed_/7/c/csm_C_elegans_adult_hermaphrodite_gonades_5c49f08a7f.jpg)
生命科学研究: 哪种显微镜相机适合您?
相机是显微镜系统的重要组成部分,对系统的性能有重大影响。在选择相机时,重要的是不仅要看技术规格,还要考虑您的样品、技术、对比方法以及您希望获得的数据类型。
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![[Translate to chinese:] Branched organoid growing in collagen where the Nuclei are labeled blue. To detect the mechanosignaling process, the YAP1 is labeled green. [Translate to chinese:] Branched organoid growing in collagen where the Nuclei are labeled blue. To detect the mechanosignaling process, the YAP1 is labeled green.](/fileadmin/_processed_/a/e/csm_Branched_organoid_growing_in_collagen_dc289aa8c6.jpg)
检查癌症类器官的发展进程
德国慕尼黑工业大学的Andreas Bausch实验室研究细胞和生物体中不同结构和功能形成的细胞和生物物理机制。他的团队设计了新的策略、方法和分析工具,以量化微米和纳米等级的发展机制和动态过程。关键研究领域包括干细胞和类器官,从乳腺类器官到胰腺癌类器官,以更好地了解疾病模型。