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![[Translate to chinese:] Camera image during auto alignment. The feedback lines indicate if the correct edges in the image are detected. Green: Vertical center line; Magenta: Upper edge of the light gap; White: Lower edge of the light gap (not visible here, falling together with red line); Red: Knife edge; Blue: Left and right edge of the block face being automatically detected.](/fileadmin/_processed_/d/1/csm_Camera_image_during_auto_alignment_UC_Enuity_5e6b7b9057.jpg "[Translate to chinese:] Camera image during auto alignment. The feedback lines indicate if the correct edges in the image are detected. Green: Vertical center line")
高质量超薄切片：样品与切片刀自动对齐
超薄切片技术是获取样品切片的最常用方法。在室温条件制备时，将样品小块嵌入环氧树脂中，然后通过修剪去除多余的树脂，并使用玻璃刀或金刚石刀将样品切成厚度为50-100纳米之间的薄片。
![[Translate to chinese:] Section ribbons with increasing section thickness - silver to purple ending in blue sections.](/fileadmin/_processed_/7/2/csm_Section_ribbons_with_increasing_section_thickness_d0e10995bf.jpg "[Translate to chinese:] Section ribbons with increasing section thickness - silver to purple ending in blue sections.")
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![[Translate to chinese:] Particulate contamination in between moving metal plates.](/fileadmin/_processed_/b/d/csm_Particulate_contamination_in_between_moving_metal_plates_2_3571994896.jpg "[Translate to chinese:] Particulate contamination in between moving metal plates.")
高效清洁度分析的关键因素
在汽车和电子行业，零部件上细小的污染颗粒物也可能影响产品的性能，导致产品出现故障，或使用寿命缩短。对于汽车来说，过滤系统很容易受到影响。对于电子产品来说，印刷电路板（PCB）或连接器上的污染可能会导致短路。因此，清洁度在现代制造业的质量控制中占有核心地位，特别是使用由不同供应商生产的部件时，更要重点关注清洁情况。车辆或设备的关键部件如果受到污染，整个系统就可能发生故障。因此，高效清洁度分析过程必须…
![[Translate to chinese:] Images of the same area of a processed wafer taken with standard (left) and oblique (right) brightfield illumination using a Leica compound microscope. The defect on the wafer surface is clearly more visible with oblique illumination.](/fileadmin/_processed_/9/1/csm_Processed_wafer_standard_and_oblique_brightfield_illumination_556d919235.jpg "[Translate to chinese:] Images of the same area of a processed wafer taken with standard (left) and oblique (right) brightfield illumination using a Leica compound microscope. The defect on the wafer surface is clearly more visible with oblique illuminati")
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![[Translate to chinese:] Preparation of an IC-chip cross section: grinding and polishing of the chip cross section.](/fileadmin/_processed_/3/b/csm_Grinding_polishing_EM_TXP_81f93bc20c.jpg "[Translate to chinese:] Preparation of an IC-chip cross section: grinding and polishing of the chip cross section.")
电子产品制造截面分析
本文将讨论印刷电路板 (PCB) 和总成 (PCBA)、集成电路 (IC) 和电池组件的横截面为什么对质量控制 (QC)、故障分析 (FA) 和研发 (R&D) 有效，以及如何制备这些横截面。
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采用徕卡THUNDER-DM6B观察SARS-CoV-2感染宿主细胞及其复制过程
冠状病毒2致重度急性呼吸综合征(SARS-CoV-2)
冠状病毒2致重度急性呼吸综合征(SARS-CoV-2)出现于2019年末，并快速传播全世界。由于其大面积的影响，研究人员对病毒的性质进行了深入的研究以期最终阻止大流行。一个重要的方面是病毒如何在宿主细胞中复制。Ogando及其同事的研究已经揭示了SARS-CoV-2的复制动力学、适应能力和细胞病理学。他们的工具之一是用荧光显微镜观察SARS…
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![[Translate to chinese:] Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.](/fileadmin/_processed_/0/6/csm_Fluorescence_microscopy_image_of_liver_tissue_0748f2a4d5.jpg "[Translate to chinese:] Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.")
落射荧光显微镜和反射对比显微镜
多年来，荧光显微镜一直仅使用透射光和暗场照明。随着时间的推移，对改进照明的需求不断增长，这导致了落射照明（也称为入射光照明）的发展。经过 40 年的发展和改进，落射照明荧光显微镜已成为生命科学、临床医学诊断和材料科学领域常规实验室工作和研究的实用方法。大部分开发工作由 Ploem 集团和 Leitz 公司（现为 Leica Microsystems）完成。
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![[Translate to chinese:] Material sample with a large height, size, and weight being observed with an inverted microscope.](/fileadmin/_processed_/f/4/csm_Inverted_microscope_with_large_sample_crop_9ca60cecc7.jpg "[Translate to chinese:] Material sample with a large height, size, and weight being observed with an inverted microscope.")
工业应用中倒置显微镜相较于正置显微镜的五大优势
使用倒置显微镜时，您需要从下方观察样本，因为倒置显微镜的光学元件位于样本下方，而使用正置显微镜时，您需要从上方观察样本。一直以来，倒置显微镜主要用于生命科学研究，因为重力将样本沉入含有水性溶液的托座底部，从上方则无法观察到太多内容。但近段时间以来，倒置显微镜在工业应用中也变得越来越流行。我们现在一起来了解倒置显微镜在工业应用中的优势。
