Leica Microsystems: 显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

用激光显微切割改进 RNA 分析

帕金森病是一种与大脑多巴胺释放神经元细胞死亡相关的进行性神经退行性疾病。疾病患者和健康个体之间多巴胺释放神经元基因表达差异允许定义靶基因。对于 RNA 分析，单细胞分辨率至关重要。分析混合多巴胺释放神经元和其他细胞会扭曲结果。可以通过激光显微切割（LMD）分离和分析组织中的单个多巴胺能神经元。由于不均匀的细胞群体而导致的误解被排除。

Comparison when observing a rodent model organism with a Greenough versus CMO (common main objective) stereo microscope for a task like surgery.

Rodent and Small-Animal Surgery

Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.

HeLa cells stimulated with LPS. Image has been subjected to deconvolution.

Chronic Inflammation Under the Microscope

In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…

CARS image of cellulose fibers. The fibers are visualized through the C–H vibrations of the polyglucan chains in cellulose.

CARS Microscopy: Imaging Characteristic Vibrational Contrast of Molecules

Coherent anti-Stokes Raman scattering (CARS) microscopy is a technique that generates images based on the vibrational signatures of molecules. This imaging methods does not require labeling, yet…