![[Translate to chinese:] John B. Gurdon](/fileadmin/_processed_/0/f/csm_John_B_Gurdon_dae2e40438.jpg "[Translate to chinese:] John B. Gurdon")
2012年诺贝尔生理学或医学奖——干细胞研究
诺贝尔奖表彰了这两位科学家，他们发现成熟、分化的细胞可以被重编程为能够发育成身体所有组织的未成熟具有干性的细胞。他们的发现彻底改变了我们对细胞和生物体发育过程的理解。
Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
活细胞成像简介
了解复杂且快速变化的细胞动力学是深入探索生物进程的重要一步。因此，现代生命科学研究越来越需要关注于在分子水平上实时发生的生理事件。
Applications of TIRF Microscopy in Life Science Research
The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or…
Total Internal Reflection Fluorescence (TIRF) Microscopy
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
![[Translate to chinese:] Snapshot from a time lapse of a calcium imaging experiment using the ratiometric calcium indicator Fura-2.](/fileadmin/_processed_/5/5/csm_Time_lapse_of_calcium_imaging_experiment_using_the_ratiometric_calcium_indicator_Fura-2_d9ca75ab1a.jpg "[Translate to chinese:] Snapshot from a time lapse of a calcium imaging experiment using the ratiometric calcium indicator Fura-2.")
比例成像
细胞的许多基本功能在很大程度上依赖于离子（例如钙、镁）、电压势和细胞质与周围细胞外空间之间的 pH 值的微妙但动态的平衡。这些平衡的变化会显著改变细胞的行为和功能。因此，实时测量细胞内离子、电压和 pH…
Optical Contrast Methods
Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
电生理学和深层组织成像的新标准
神经和肌肉细胞的功能依赖于通过离子通道流动的离子电流。这些离子通道在细胞生理过程中起着关键作用。研究离子通道的一种方法是使用膜片钳技术。这种方法可以详细研究离子通道，并记录不同类型细胞（主要是神经元、肌纤维或胰岛β细胞）的电活动。膜片钳技术由Erwin Neher和Bert…
