Fast, High-quality Vitrification with the EM ICE High Pressure Freezer
The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…
![[Translate to chinese:] Cryo FIB lamella - Overlay of SEM and confocal fluorescence image. Target structure in yeast cells (nuclear pore proteine Nup159-Atg8-split Venus, red) marked by an arrow. Scale bar: 5 µm. Alegretti et al., Nature 586, 796-800 (2020).](/fileadmin/_processed_/c/d/csm_Targeting_Nuclear_Pore_Complexes_teaser_27b8f39513.jpg "[Translate to chinese:] Cryo FIB lamella - Overlay of SEM and confocal fluorescence image")
使用冷冻共聚焦显微镜定位活性循环核孔复合物
本文介绍了如何利用冷冻光学显微镜，尤其是冷冻共焦显微镜来提高冷冻工作流程的可靠性。评估了EM网格和样品的质量，并分析了目标结构的分布。本文展示了如何将冷冻共焦3D数据投射到SEM图像上，将感兴趣结构可靠地保留在FIB切割的薄片内，以便在冷冻TEM中进行进一步研究。
Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…
Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own…
High-pressure freezing: Revealing functional mechanisms of synaptic transmission
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…
Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…
钢材中非金属夹杂物评级标准相关的常见问题
向全球用户供应单批次钢材组件和产品时，产品需满足多种钢材质量标准。这些用户需求成为了摆在供应商面前的一道难题。由于夹杂物对钢材质量有显著影响，各种标准都对非金属夹杂物评级规定了严格的评级方法。钢材质量评级有助于确保产品和组件满足不同的性能和安全规格要求。然而，由于国际、区域和组织性的标准繁多，而且这些标准还会不断更新变化，因此单批次钢材组件/产品要满足多种钢材质量标准的要求是一项难度不小的挑战。为…
![[Translate to chinese:] Image source: shutterstock](/fileadmin/_processed_/0/b/csm_Steelworks_c791b5866d.jpg "[Translate to chinese:] Image source: shutterstock")
分析钢铁中的非金属夹杂物
我们常常发现自己陷入了通过标线和比较图进行繁琐的分析，根据多个标准进行耗时的双重评估或来自不同用户的带有偏见的主观检查结果。
在本次网络研讨会中，Nicol Ecke 博士将讨论使用 LAS X 钢铁专家 自动分析非金属夹杂物的优势。了解这将如何帮助您比以往更快、更轻松地获得您想要的可靠、公正且符合标准的结果。
EM TIC 3X进行离子束刻蚀简介
在这篇文章中，您可以了解到如何通过使用EM TIC 3X离子束研磨仪的离子束蚀刻工艺来优化样品的制备质量。文中简介了EM TIC 3X仪器特性，以此解释如何灵活地将该设备应用于各类研究领域的样本制备工作中。本文将帮助读者了解离子束刻蚀工艺的基本原理，及其如何在各种应用中获得高分辨率的SEM图像。本文也将介绍EM TIC…
