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荧光活细胞成像技术
理解复杂和/或快速的细胞动力学是探索生物过程的重要一步。因此,如今的生命科学研究越来越关注动态过程,例如细胞迁移,细胞、器官或整个动物的形态变化,以及活体样本中的实时生理事件(如细胞内离子成分的变化)。
满足此类高难度需求的一种方法是采用某些统称为活细胞成像的光学方法。
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显微镜在病毒学中的应用
引起新型冠状病毒肺炎(Covid-19)的冠状病毒SARS-CoV-2肆虐全球并影响了我们生活的方方面面。对于免疫和治疗方法的搜索研究(即如何抗击该病毒)成为了2020年全人类的第一要务。显微镜在这类研究中起着重要作用。为了了解受体结合、基因组释放、复制、装配和病毒出芽的基本原理以及我们的免疫系统效应,可以使用不同的方法和显微镜。本文概述了为什么显微镜是病毒学和感染生物学的重要工具,并举例说明了不…
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Universal PAINT – Dynamic Super-Resolution Microscopy
Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…
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Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
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Applications of TIRF Microscopy in Life Science Research
The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or…
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Total Internal Reflection Fluorescence (TIRF) Microscopy
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…