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![[Translate to chinese:] Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM. [Translate to chinese:] Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM.](/fileadmin/_processed_/2/7/csm_Tilescan_of_brain_organoid_section_46d510ba4e.jpg)
研究大脑健康的成像类器官模型
小胶质细胞是特化的脑驻留免疫细胞,在大脑发育、平衡和疾病中发挥着至关重要的作用。然而,到目前为止,模拟人脑环境与小胶质细胞之间相互作用的能力还非常有限。
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![[Translate to chinese:] Microscopy for neuroscience research [Translate to chinese:] Microscopy for neuroscience research](/fileadmin/_processed_/9/5/csm_Microscopy_for_neuroscience_research_cc303ca353.jpg)
神经科学显微镜面临哪些挑战?
显微镜是神经科学研究领域的强大工具。不过,当涉及到对神经过程进行成像以及使用不同的样品类型(例如厚神经组织或脑类器官)时,科研人员可能会面临到很多挑战。这本30页的电子书包含众多真实的案例,以讨论我们最常见到的一些挑战,同时展示了如何使用THUNDER 成像技术克服这些挑战。
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![](/fileadmin/_processed_/3/7/csm_Getting_sharper_3D_images_teaser_dd8f41d6b0.jpg)
超越反卷积
宽场荧光显微镜通常用于视觉呈现生命科学样本中的结构并获取重要信息。利用荧光蛋白或染料,以高度特异性的方式标记离散的样本部分。为了充分了解某种结构,可能需要以三维方式呈现,但这会对使用显微镜带来某些挑战。
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![Mouse whole-mount retina. Image courtesy of the Experimental Ophthalmology Group, University of Murcia, Spain. Mouse whole-mount retina. Image courtesy of the Experimental Ophthalmology Group, University of Murcia, Spain.](/fileadmin/_processed_/4/a/csm_Mouse_whole-mount_retina_Thundered_abe5e38481.jpg)
快速、高灵敏度成像和人工智能辅助分析
The specificity of fluorescence microscopy allows researchers to accurately observe and analyze biological processes and structures quickly and easily, even when using thick or large samples. However,…
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![3D reconstruction of an isolated human islet 3D reconstruction of an isolated human islet](/fileadmin/_processed_/d/1/csm_THUNDER-Imager-3D-Cell-Culture_isolated-human-islet_6bcb590f44.jpg)
为活细胞成像创造新选择
对厚实的活体样本进行成像时,主要挑战之一是获得图像质量与组织完整性之间的平衡。长时间的图像采集期间,弱信号光会导致低信号水平,导致图像对比度低以及分割和分析困难。需要通过高剂量成像或高时间分辨率成像技术加强信号强度时,这一问题更加突出。一个常见问题是:我如果快速成像、一次完成,会不会造成样本过度漂白或者细胞死亡?
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![Raw widefield and THUNDER image of a mouse dorsal root ganglion with tdTomato (red) expressed in the sensory neurons. Raw widefield and THUNDER image of a mouse dorsal root ganglion with tdTomato (red) expressed in the sensory neurons.](/fileadmin/_processed_/4/3/csm_Mouse_dorsal_root_ganglion_sensory_neurons_THUNDER_teaser_86235c43e7.jpg)
感觉神经元的高对比度快速三维成像
本文讨论了相比传统的宽场显微镜,使用large volume computational clearing(LVCC)技术的THUNDER组织成像系统如何获取背根神经节(DRG)组织高对比度的快速三维成像图,获得感觉神经元更为清晰的解析图像。神经科学研究的一项主要领域集中在感觉神经元对触觉和痛觉的影响方面。深入理解这种现象对于神经系统疾病和疗法的发展具有重要意义。
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![Blood vessels imaged with THUNDER & Aivia Left-hand image: The distribution of immune cells (white) and blood vessels (pink) in white adipose tissue (image captured using the THUNDER Imager 3D Cell Culture). Right-hand image: The same image after automated analysis using Aivia, with each immune cell color-coded based on its distance to the nearest blood vessel. Image courtesy of Dr. Selina Keppler, Munich, Germany.](/fileadmin/_processed_/f/a/csm_Fat_tissue_THUNDER_THUNDER-Aivia_teaser_e142629a8c.jpg)
精确分析宽视野荧光图像
利用荧光显微镜的特异性,即便是使用厚样品和大尺寸样品,研究人员也能够快速轻松地准确观察和分析生物学过程和结构。然而,离焦荧光会提高背景荧光,降低对比度,影响图像的精确分割。THUNDER 与Aivia 的组合可以有效解决这一问题。前者可以消除图像模糊,后者会使用人工智能技术自动分析宽视野图像,提高操作速度和精确性。下面,我们来详细了解下这一协作方法。
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![Fluorescently stained mouse tibia, imaged with a THUNDER Imager 3D Tissue. Courtesy of Dr. Anjali Kusumbe, University of Oxford, UK.](/fileadmin/academy/2021/Abstracts_Interviews_Webinars/High-resolution_3D_imaging_to_investigate_tissue_ageing_teaser.jpg)
High-resolution 3D Imaging to Investigate Tissue Ageing
Award-winning researcher Dr. Anjali Kusumbe demonstrates age-related changes in vascular microenvironments through single-cell resolution 3D imaging of young and aged organs.