Leica Microsystems: 显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

[Translate to chinese:] Dividing fission yeast S. pombe stained with two markers against spindle pole bodies (Pcp1-GFP, green) and cytokinesis ring (Rlc1-mCherry; red).

研究细胞分裂

细胞分裂研究对于科学家更好地了解生物体的生长、增殖和繁殖等现象非常重要。本文展示了如何利用THUNDER成像仪获取的裂变酵母活细胞的清晰三维图像来研究细胞环和纺锤极体等亚细胞结构。

[Translate to chinese:] Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA. Images courtesy of Dr. Reyna Martinez – De Luna, Upstate Medical University, Department of Ophthalmology.

自适应反卷积与 Computational Clearing 结合的力量

反卷积是一种计算方法，用于恢复被点扩散函数（PSF）和噪声源破坏的物体图像。在本技术简介中，您将了解徕卡显微系统提供的反卷积算法如何帮助您克服宽视场 (WF) 荧光显微镜中由于光的波动性和光学元件对光的衍射而造成的图像分辨率和对比度损失。探索由用户控制或自动反卷积的方法，查看并解析更多的结构细节。

改进成像技术以了解细胞器膜细胞动态

了解正常组织和肿瘤组织中的细胞功能，是推动潜在治疗策略研究和了解某些治疗失败原因的关键因素。单细胞分析在生物医学研究中至关重要，它能揭示在癌症等复杂疾病中哪些细胞和分子通路发生了改变。

Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture. Image courtesy of Dr. Selina Keppler, Munich, Germany.

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…

从器官到组织再到细胞：使用宽场显微镜分析 3D 标本

在传统的宽场显微镜下从厚的三维样本中获取高质量的数据和图像是具有挑战性的，因为存在失焦光的干扰。在本次网络研讨会中，Falco Krüger 展示了THUNDER成像仪如何通过Computational Clearing技术使这一切成为可能。

Mouse retina was fixed and stained by following reagents: anti-CD31 antibody (green): Endothelia cells, IsoB4 (red): Blood vessels, and microglia anti-GFAP antibody (blue): Astrocytes Sample courtesy by Jeremy Burton, PhD and Jiyeon Lee, PhD, Genentech Inc., South San Francisco, USA. Imaged by Olga Davydenko, PhD (Leica). Imaged with a THUNDER Imager 3D Cell Culture.

An Introduction to Computational Clearing

Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…

[Translate to chinese:] Influenca in lung epithelial cells (porcine) - THUNDER Imager 3D Cell Culture Influenca virus – red, cilia – green, Nuclei – blue.

免疫荧光如何帮助病毒学研究？

由于全球 COVID-19 大流行，现代病毒学研究变得比以往任何时候都更加重要。病毒学家可以应用许多强大的技术和检测方法来研究病毒的结构和功能。