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将动态活细胞数据融入超微结构
采用徕卡Nano的工作流程,可以避免过去如海底捞针似的寻找。利用光电关联显微技术,在适当的时间直接鉴别出正确的细胞,并将动态的活细胞数据融入其超微结构中。
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![[Translate to chinese:] Dividing fission yeast S. pombe stained with two markers against spindle pole bodies (Pcp1-GFP, green) and cytokinesis ring (Rlc1-mCherry; red). [Translate to chinese:] Dividing fission yeast S. pombe stained with two markers against spindle pole bodies (Pcp1-GFP, green) and cytokinesis ring (Rlc1-mCherry; red).](/fileadmin/_processed_/a/7/csm_Studying_Cell_Division_teaser_662fd7ea24.jpg)
研究细胞分裂
细胞分裂研究对于科学家更好地了解生物体的生长、增殖和繁殖等现象非常重要。本文展示了如何利用THUNDER成像仪获取的裂变酵母活细胞的清晰三维图像来研究细胞环和纺锤极体等亚细胞结构。
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![[Translate to chinese:] Image: Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA. [Translate to chinese:] Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA. Images courtesy of Dr. Reyna Martinez – De Luna, Upstate Medical University, Department of Ophthalmology.](/fileadmin/_processed_/3/a/csm_The_Power_of_Pairing_Adaptive_Deconvolution_teaser_5d4bdbe29b.jpg)
自适应反卷积与 Computational Clearing 结合的力量
反卷积是一种计算方法,用于恢复被点扩散函数(PSF)和噪声源破坏的物体图像。在本技术简介中,您将了解徕卡显微系统提供的反卷积算法如何帮助您克服宽视场 (WF) 荧光显微镜中由于光的波动性和光学元件对光的衍射而造成的图像分辨率和对比度损失。探索由用户控制或自动反卷积的方法,查看并解析更多的结构细节。
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改进成像技术以了解细胞器膜细胞动态
了解正常组织和肿瘤组织中的细胞功能,是推动潜在治疗策略研究和了解某些治疗失败原因的关键因素。单细胞分析在生物医学研究中至关重要,它能揭示在癌症等复杂疾病中哪些细胞和分子通路发生了改变。
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![Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture. Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture. Image courtesy of Dr. Selina Keppler, Munich, Germany.](/fileadmin/_processed_/1/6/csm_Mouse-lymphnode_THUNDER_d0a9bfac70.jpg)
Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve…
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From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
Obtaining high-quality data and images from thick 3D samples is challenging using traditional widefield microscopy because of the contribution of out-of-focus light. In this webinar, Falco Krüger…
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![Mouse retina Mouse retina was fixed and stained by following reagents: anti-CD31 antibody (green): Endothelia cells, IsoB4 (red): Blood vessels, and microglia anti-GFAP antibody (blue): Astrocytes Sample courtesy by Jeremy Burton, PhD and Jiyeon Lee, PhD, Genentech Inc., South San Francisco, USA. Imaged by Olga Davydenko, PhD (Leica). Imaged with a THUNDER Imager 3D Cell Culture.](/fileadmin/_processed_/3/5/csm_An_Introduction_to_Computational_Clearing_Teaser_8831f2b97f.jpg)
An Introduction to Computational Clearing
Many software packages include background subtraction algorithms to enhance the contrast of features in the image by reducing background noise. The most common methods used to remove background noise…
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Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…