Leica Microsystems: 显微镜科学与教学知识中心

显微镜科学与教学知识中心

徕卡显微系统的知识库提供有关显微镜学科的科学研究和教学材料。内容旨在对显微镜初学者、有经验的显微镜操作实践者和使用显微镜的科学家在他们的日常工作和实验有所帮助。这里有探索交互式教程和应用笔记，你可以找到你需要的显微镜的基础知识以及前沿技术——快来加入徕卡显微知识社区，分享您的专业知识！

[Translate to chinese:] Pinhole diameter and diffraction pattern.

共聚焦显微镜针孔效应

在操作共聚焦显微镜，或在讨论这种装置的特性和参数时，我们不可避免地提到针孔及其直径。这篇简短的文章是针对那些没有足够时间钻研共聚焦显微镜的理论和细节但又想了解针孔效应的用户们来解释针孔的意义。

Comparison when observing a rodent model organism with a Greenough versus CMO (common main objective) stereo microscope for a task like surgery.

Rodent and Small-Animal Surgery

Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.

脑部导航

神经外科手术面临的挑战之一是在手术部位的定位。在切除肿瘤、去除动静脉畸形或夹闭动脉瘤时，外科医生常常需要在健康和功能性脑组织附近工作。在切除肿瘤时，挑战始终是尽可能保留健康组织。神经导航技术，也称为图像引导手术（IGS），使外科医生能够在切割之前规划理想的入路点，并通过提供术中定位来帮助执行该计划。

[Translate to chinese:] Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-illumination using narrow-band blue (490 nm) light. Note the increased image contrast (Ploem, 1967)

Milestones in Incident Light Fluorescence Microscopy

Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…