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利用直流磁控溅射镀膜仪改进HR-SEM用金属薄膜
制备技术(诸如使用各种涂层方法)在高分辨率扫描电子显微镜(HR-SEM)中起着重要作用。对于生物和合成样品等非导电样品,必须制备薄导电层以防止荷电。
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![Infinity port Infinity port](/fileadmin/_processed_/c/1/csm_Infinity_Port_d9f0c4837e.jpg)
无限远光学系统
“无限远光学”这一概念是指在显微镜的物镜和镜筒透镜之间具有平行光线的光束路径。平面光学元件可以进入到这个“无限远空间”中,而不影响成像,这对于利用DIC或荧光等对比度方法至关重要。
现代显微技术需要在无限远光路中添加多种光学仪器,如光源或激光装置。满足这一需求的不同方法已经出现,本文对其进行了描述。
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Imaging of Host Cell-bacteria Interactions using Correlative Microscopy under Cryo-conditions
Pathogenic bacteria have developed intriguing strategies to establish and promote infections in their respective hosts. Most bacterial pathogens initiate infectious diseases by adhering to host cells…
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Image gallery: Life Science Imaging with the Leica DVM6 Digital Microscope
Digital microscopes can be a great help in life science applications such as the documentation in botany, entomology studies and crop science, or the digitization of museum collections. The image…
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![[Translate to chinese:] Proveo lightbeam [Translate to chinese:] Proveo lightbeam](/fileadmin/_processed_/3/6/csm_proveo_lightbeam_1200x1800_81763a1d26.jpg)
使用CoAx 4 四光束同轴立体照明技术实施的白内障手术
稳定的红光反射是白内障手术所用的眼科手术显微镜的最重要功能之一。红光反射让手术医生可以观察到晶状体结构,为其安全成功地实施手术提供清晰的视野。如何能清晰的观察到晶状体结构,特别是在手术过程中的超声乳化、晶状体摘除以及人工晶状体植入等关键阶段,始终提供稳定的红光反射,是手术显微镜面临的挑战。
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Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection
During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome.…
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![A 17th-century compound microscope (© Golub Collection – University of California, Berkeley/Steven Ruzin, Curator) A 17th-century compound microscope (© Golub Collection – University of California, Berkeley/Steven Ruzin, Curator)](/fileadmin/_processed_/3/9/csm_17th-century_compound_microscope_5700831e3c.jpg)
光学显微镜简史
显微镜的历史始于中世纪。早在11世纪,阿拉伯世界就使用抛光绿柱石制成的平凸透镜作为阅读石来放大手稿。然而,将这些透镜发展成显微镜并非某一个人的功劳,而是众多科学家和学者共同努力的结果。
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From Light to Mind: Sensors and Measuring Techniques in Confocal Microscopy
This article outlines the most important sensors used in confocal microscopy. By confocal microscopy, we mean "True Confocal Scanning", i.e. the technique that illuminates and measures one single…