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Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…
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Förster Resonance Energy Transfer (FRET)
The Förster Resonance Energy Transfer (FRET) phenomenon offers techniques that allow studies of interactions in dimensions below the optical resolution limit. FRET describes the transfer of the energy…
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An Introduction to CARS Microscopy
CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which…
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Super-Resolution GSDIM Microscopy
The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within…
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Mosaic Images
Confocal laser scanning microscopes are widely used to create highly resolved 3D images of cells, subcellular structures and even single molecules. Still, an increasing number of scientists are…
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Is that Document Genuine or Fake? How do They Identify Fake Documents?
This article shows how forensic experts use microscopy for analysis to identify counterfeit, fake documents, such as ID cards, passports, visas, certificates, etc. Then they know if it is genuine or…
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金相学：色彩与相衬度分析－ 微观结构对比分析
显微组织形貌的检测在材料科学和失效分析中起着决定性的作用。在光学显微镜中有许多可能使材料的真实结构可视化。本文中显示的图像示例演示了使用的这些技术的信息及潜力。
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共聚焦光学截面厚度
共聚焦显微镜用于光学切片比较厚的样本。最直接的问题是：1. 什么是“比较厚的样本”；2. 光学切片到底有多厚？这两个问题在一定程度上是相关的，即假设一份厚样本至少比光学切片厚10倍。
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Optical Contrast Methods
Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
