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白激光
在生物医学应用中,共聚焦显微镜的完美光源它应该有足够的强度,可调谐的波长,以便同时激发一系列样品。此外,它应该成为荧光寿命实验的脉冲光源。这样的光源已经出现:白激光。它采用高能脉冲红外光纤激光器经过光子晶体光纤以产生连续光谱。通过声光调制滤片从该连续光谱中选择窄带激光。
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光学显微镜 – 基础入门
150多年来,光学显微镜一直都是生命科学研究以及材料科学当中的标准工具之一。要经济有效地使用这一工具,了解光学的基础知识,尤其是构成每一台显微镜的基本组成部分将会获得很大的帮助。
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Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
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冷发光基础原理
自然界中有许多发光过程。冷发光是一系列发光现象的统称,是指不因高温而引发的发光事件。本文描述了不同形式的冷光并对荧光进行了详细介绍。在第二部分解释了描述荧光色素的相关技术术语,如淬灭、漂白或量子效率,以便对荧光分子的基础特性有更深入的了解。
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Total Internal Reflection Fluorescence (TIRF) Microscopy
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
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Applications of TIRF Microscopy in Life Science Research
The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or…
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Ratiometric Imaging
Many fundamental functions of a cell strongly depend on delicate, but nevertheless dynamic balances of ions (e.g. calcium, magnesium), voltage potentials and pH between the cell’s cytosol and the…