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样本修块简介
使用超薄切片机制作样本超薄切片前需要预制样本。在预制过程中,必须特别留意样本尺寸(切片尺寸)、样本位置(目标定位)和块面边缘精准度。这个过程通常叫做修块,在此过程中样本大多被修块成金字塔截头锥体的形状。
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Glycerol Spraying/Platinum Low Angle Rotary Shadowing of DNA with the Leica EM ACE600 e-beam
Glycerol spraying/low angle rotary shadowing (Aebi and Baschong, 2006) is a preparation technique used in biology to visualize structures yielding insufficent contrast with other techniques, due to…
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比对显微镜 125 年的发展历程
要科学准确地对两个物体进行光学比较,必须能够同时观察它们。特别是在比较只能借助光学放大系统才能观察到的微小物体时,更是如此。如果你只有一台显微镜,而且必须交替观察两个物体,那么你就需要有出色的记忆力,而且永远不能排除判断错误的风险,尤其是在检查结构、颜色或轮廓只有细微差别的物体时。这种认识促使圣彼得堡的地质学教授亚历山大-冯-伊诺斯特兰泽夫(Alexander von…
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Preparation of Laser Microdissected Plant Cell and Tissue Material for Microchemical Analysis
Secondary plant metabolites are involved in various plant physiological and biochemical processes, allowing plants to interact successfully with their biotic and abiotic environment. Distribution…
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Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips
The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…
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冷冻替代的简要介绍
冷冻替代是一种在足够低温的条件下进行的脱水过程,以避免形成冰晶,并有效规避在环境温度下脱水后观察到的破坏性影响。在冷冻替代期间,“冷冻”水被有机溶剂溶解,有机溶剂通常也含有化学固定剂。冷冻替代与细胞成分的即刻物理固定(冷冻固定)以及树脂包埋相关联。一旦替代完成,样品会逐渐升温,并与常规制备的样品一样进行进一步处理。与化学固定技术相比,成功的冷冻固定及后续FS处理显示出优良的精细结构保存[2]。即使…
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声光调制在全光谱型激光共聚焦显微镜系统的应用
荧光最显著的特征是照射光(激发光)和检测光(发射光)颜色之间的偏移,称为斯托克斯位移。因此,在荧光成像中,不仅要将激发光和发射光的相应波长过滤出来,还需要将激发光从发射光中分离。过去,通常用平面光学元件,包括灰色或彩色滤光片和反射镜进行滤光和分光。虽然有多种平面光学元件可供使用,但固定的规格和低切换效率使其在使用上具有局限性,并且采用不同角度或梯度的涂层作为激发光和发射光的调谐方法也被证实并不可行…
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Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic
On October 7th 2013, The Nobel Assembly at Karolinska Institutet has decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof…
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Thermodynamic Considerations Regarding the LN2 in a High Pressure Freezer
Employing liquid nitrogen (LN2) as a coolant in the complex process of high pressure freezing raises certain considerations regarding phase transition not only of the liquid sample to be frozen but…