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![[Translate to chinese:] Camera image during auto alignment. The feedback lines indicate if the correct edges in the image are detected. Green: Vertical center line; Magenta: Upper edge of the light gap; White: Lower edge of the light gap (not visible here, falling together with red line); Red: Knife edge; Blue: Left and right edge of the block face being automatically detected.](/fileadmin/_processed_/d/1/csm_Camera_image_during_auto_alignment_UC_Enuity_5e6b7b9057.jpg "[Translate to chinese:] Camera image during auto alignment. The feedback lines indicate if the correct edges in the image are detected. Green: Vertical center line")
高质量超薄切片：样品与切片刀自动对齐
超薄切片技术是获取样品切片的最常用方法。在室温条件制备时，将样品小块嵌入环氧树脂中，然后通过修剪去除多余的树脂，并使用玻璃刀或金刚石刀将样品切成厚度为50-100纳米之间的薄片。
![[Translate to chinese:] Section ribbons with increasing section thickness - silver to purple ending in blue sections.](/fileadmin/_processed_/7/2/csm_Section_ribbons_with_increasing_section_thickness_d0e10995bf.jpg "[Translate to chinese:] Section ribbons with increasing section thickness - silver to purple ending in blue sections.")
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![[Translate to chinese:] Multicolor fixed STED image. Inner ear section, mouse, TauSTED Xtend 589 on AF488 and TauSTED Xtend 775 on AF633-Phalloidin. Sample courtesy of Dennis Derstrof, Klinik für Hals-, Nasen und Ohrenheilkunde, Universität Marburg & Prof. Dr. Dominik Oliver aus dem Institut für Physiologie und Pathophysiologie, Abteilung für Neurophysiologie, Universität Marburg.](/fileadmin/_processed_/c/a/csm_Inner_ear_section_mouse_multicolor_fixed_TauSTED_Xtend_09ebb898fd.jpg "[Translate to chinese:] Multicolor fixed STED image. Inner ear section, mouse, TauSTED Xtend 589 on AF488 and TauSTED Xtend 775 on AF633-Phalloidin.")
细胞活成像的纳米级扩展
新的STED显微技术方法——TauSTED Xtend，使得在纳米级别下对活体完整样本进行扩展多色成像成为可能。通过结合空间和寿命信息，TauSTED Xtend提供了额外一层信息，允许在极低的光剂量下分辨小细节并在整体结构中解析它们。
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![[Translate to chinese:] Multicolor TauSTED Xtend 775 for Cell Biology applications that require nanoscopy resolution for multiple cellular components. Cells showing vimentin fibrils (AF 594), actin network (ATTO 647N), and nuclear pore basket (CF 680R). Sample courtesy of Brigitte Bergner, Mariano Gonzales Pisfil, Steffen Dietzel, Core Facility Bioimaging, Biomedical Center, Ludwig-Maximilians-University, Munich, Germany.](/fileadmin/_processed_/5/4/csm_Triple_color_fixed_sample_TauSTED_Xtend_2_3_695f805a53.jpg "[Translate to chinese:] Multicolor TauSTED Xtend 775 for Cell Biology applications that require nanoscopy resolution for multiple cellular components. Cells showing vimentin fibrils (AF 594), actin network (ATTO 647N), and nuclear pore basket (CF 680R).")
STED样品制备指南
这份指南旨在帮助用户优化受激发射损耗（STED）纳米成像的样品制备，特别是在使用徕卡微系统的STED显微镜时。它提供了单色STED成像用荧光标记的概述，并对其性能进行了评级。
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How to Streamline Your Histology Workflows
Streamline your histology workflows. The unique Fluosync detection method embedded into Mica enables high-res RGB color imaging in one shot.
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How to Prepare Samples for Stimulated Raman Scattering (SRS) imaging
Find here guidelines for how to prepare samples for stimulated Raman scattering (SRS), acquire images, analyze data, and develop suitable workflows. SRS spectroscopic imaging is also known as SRS…
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加速不同组织多重成像的发现
组织的多重成像对于肿瘤-免疫相互作用的研究以及人类细胞图谱等发现工作越来越重要。 欢迎加入我们的演讲，Andrea J. Radtke 博士解释了如何使用迭代漂白扩展多重性 (IBEX) 绘制组织图谱，并讨论了用于多重成像的广泛社区资源。
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![[Translate to chinese:] Particulate contamination in between moving metal plates.](/fileadmin/_processed_/b/d/csm_Particulate_contamination_in_between_moving_metal_plates_2_3571994896.jpg "[Translate to chinese:] Particulate contamination in between moving metal plates.")
高效清洁度分析的关键因素
在汽车和电子行业，零部件上细小的污染颗粒物也可能影响产品的性能，导致产品出现故障，或使用寿命缩短。对于汽车来说，过滤系统很容易受到影响。对于电子产品来说，印刷电路板（PCB）或连接器上的污染可能会导致短路。因此，清洁度在现代制造业的质量控制中占有核心地位，特别是使用由不同供应商生产的部件时，更要重点关注清洁情况。车辆或设备的关键部件如果受到污染，整个系统就可能发生故障。因此，高效清洁度分析过程必须…
