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![[Translate to chinese:] Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light. [Translate to chinese:] Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.](/fileadmin/_processed_/0/6/csm_Fluorescence_microscopy_image_of_liver_tissue_0748f2a4d5.jpg)
落射荧光显微镜和反射对比显微镜
多年来,荧光显微镜一直仅使用透射光和暗场照明。随着时间的推移,对改进照明的需求不断增长,这导致了落射照明(也称为入射光照明)的发展。经过 40 年的发展和改进,落射照明荧光显微镜已成为生命科学、临床医学诊断和材料科学领域常规实验室工作和研究的实用方法。大部分开发工作由 Ploem 集团和 Leitz 公司(现为 Leica Microsystems)完成。
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![[Translate to chinese:] Esophageal tissue with a squamous cell carcinoma labelled with the 4 biomarkers PanCk, DAPI, NaKATPase, and Vimentin. [Translate to chinese:] Esophageal tissue with a squamous cell carcinoma labelled with the 4 biomarkers PanCk, DAPI, NaKATPase, and Vimentin.](/fileadmin/_processed_/7/1/csm_Esophageal_Squamous_Cell_Carcinoma_4_Markers_7ac3f29e4b.jpg)
探索多重生物成像如何推进癌症研究
观看行业和学术专家进行的内容丰富的讨论,分享他们在研究中使用多重成像技术的知识。了解多重成像技术如何通过发现以前难以捉摸的分子洞察力,彻底改变肿瘤学、神经学和免疫学。利用先进的成像技术深入了解组织微环境,从而对代谢紊乱和癌症等疾病有新的认识。
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![[Translate to chinese:] Material sample with a large height, size, and weight being observed with an inverted microscope. [Translate to chinese:] Material sample with a large height, size, and weight being observed with an inverted microscope.](/fileadmin/_processed_/f/4/csm_Inverted_microscope_with_large_sample_crop_9ca60cecc7.jpg)
工业应用中倒置显微镜相较于正置显微镜的五大优势
使用倒置显微镜时,您需要从下方观察样本,因为倒置显微镜的光学元件位于样本下方,而使用正置显微镜时,您需要从上方观察样本。一直以来,倒置显微镜主要用于生命科学研究,因为重力将样本沉入含有水性溶液的托座底部,从上方则无法观察到太多内容。但近段时间以来,倒置显微镜在工业应用中也变得越来越流行。我们现在一起来了解倒置显微镜在工业应用中的优势。
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共聚焦多色成像在癌症研究和免疫学中的潜力
在本次网络研讨会上,来自莫纳什制药科学研究所的CameronNowell和他的同事将分享他们在多重成像方面的经验,以及他们通过巧妙的共聚焦成像采集和利用FLIM等其他多重成像模式所取得的成果。
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![[Translate to chinese:] Serious Game in Intraoperative Neurosurgery. Image courtesy of Dr. Lucas Troude. [Translate to chinese:] Serious Game in Intraoperative Neurosurgery. Image courtesy of Dr. Lucas Troude.](/fileadmin/_processed_/b/3/csm_Serious_Game_in_Intraoperative_Neurosurgery_604083c2ec.jpg)
加强神经外科教学
模拟训练在神经外科教学中发挥着越来越重要的作用。作为徕卡神经可视化峰会(Leica Neurovisualization Summit)的一部分,Lucas Troude 博士在独家网络研讨会上介绍了术中神经外科严肃游戏(SGION)。
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![[Translate to chinese:] Acrylonitrile butadiene styrene (ABS) stained with osmium tetroxide (OsO4), sectioned with a DIATOME diamond knife at room temperature, and then imaged with HAADF TEM. [Translate to chinese:] Acrylonitrile butadiene styrene (ABS) stained with osmium tetroxide (OsO4), sectioned with a DIATOME diamond knife at room temperature, and then imaged with HAADF TEM.](/fileadmin/_processed_/a/b/csm_ABS_stained_with_OsO4_and_sectioned_with_a_DIATOME_diamond_knife_81383073f3.jpg)
用于材料切片的超薄切片技术
了解这些用于材料切片的超薄切片技术的宝贵见解,并学习如何克服在处理聚合物、金属和脆性材料时遇到的挑战。千万不要错过这个提高您对先进显微镜样品制备方法的认识的机会。