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Coherent Raman Scattering Microscopy Publication List
CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…
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DIVE Multiphoton Microscope Image Gallery
Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…
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Laser Beam Shaping for Multicolor Multiphoton Microscopy
Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely…
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![[Translate to chinese:] CARS image of cellulose fibers. The fibers are visualized through the C–H vibrations of the polyglucan chains in cellulose. [Translate to chinese:] CARS image of cellulose fibers. The fibers are visualized through the C–H vibrations of the polyglucan chains in cellulose.](/fileadmin/_processed_/b/1/csm_Fig3_02_02_2b89000fd2.jpg)
CARS 相干反斯托克斯拉曼散射显微镜: 分子特征振动对比成像
相干反斯托克斯拉曼散射(CARS)显微技术是一种根据分子振动特征生成图像的技术。这种成像方法不需要标记,但可以从一系列重要的生物分子化合物中获得特定的分子信息。
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CARS 相干反斯托克斯拉曼散射显微镜简介
共聚焦和多光子成像技术仍然是对生物样本进行复杂研究的首选方法。这些技术可将生物样本中的典型结构或动态过程可视化,并依赖于样本中现有的自发荧光物质或合适的荧光染料。传统染色方法的缺点显而易见:标记耗时,染料会随时间褪色。此外,染料会失去强度并改变样本。染料通常会产生光毒性,对样本造成伤害,进而影响实验结果。CARS(相干反斯托克斯拉曼散射)显微镜是一种无需染料的方法,它通过显示结构分子的内在振动对比…