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![[Translate to chinese:] Schematic graph of the light path in a Spalt-Ultramikroskop. [Translate to chinese:] Schematic graph of the light path in a Spalt-Ultramikroskop.](/fileadmin/_processed_/b/7/csm_Light_path_in_a_Spalt-Ultramikroskop_02c62ceafd.jpg)
共聚焦成像和光片成像
光学成像仪器可以放大微小物体,聚焦遥远星体,揭示肉眼看不见的细节。但是,它有一个众所周知且令人烦恼的问题:景深有限。我们的眼睛(也是一种光学成像装置)也有同样的困扰,但我们的大脑在信号到达意识认知之前会巧妙地移除所有不在焦点上的信息。
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Image Processing for Widefield Microscopy
Fluorescence microscopy is a modern and steadily evolving tool to bring light to current cell biological questions. With the help of fluorescent proteins or dyes it is possible to make discrete…
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![[Translate to chinese:] Scheme of a 2D mosaic scan. Drosophila melanogaster (eye section) [Translate to chinese:] Scheme of a 2D mosaic scan. Drosophila melanogaster (eye section)](/fileadmin/_processed_/e/b/csm_Mosaic_Intro_04_c164a21165.jpg)
马赛克图片
共焦激光扫描显微镜被广泛用于生成细胞、亚细胞结构甚至单分子的高分辨率三维图像。不过,越来越多的科学家正将生物研究的重点从单细胞研究扩展到整个器官和生物体,分析动物体内复杂的相互作用。