High-Pressure Freezing Protocols for Ultrastructural 3D EM
High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…
Ultramicrotome UC Enuity in Practice: Stable 15 nm Sections at ZFE
After using the UCT and UC6 ultramicrotomes, Claudia Mayrhofer calls UC Enuity a leap in stability—so robust that vibrations and temperature shifts don’t spoil sections, even with multiple users. Auto…
消除激光显微切割中的静电干扰
激光显微切割（LMD）中的静电荷会导致两种严重问题：样品粘附在带电表面而丢失，或者样品飞入相邻的孔中造成交叉污染。我们在 Leica LMD7 环境室中集成了一个离子发生器除静电。离子发生器将静电位移从 16%（16/100 次切割）降低到 0.2%（1/450+ 次切割）。低吸附 384 孔板的收集率从 65-75% 提高到 85-95%。
History, Developments and Trends of Microscopy in Cancer Research
Cancer is a global disease, with 18 million new cases diagnosed and 10 million cancer-related deaths worldwide in 2020. This burden is set to increase, with a projected increase in cases of ~55% by…
荧光染料应用和特性概述
本文将介绍常用的荧光染料并概述其特性。荧光显微镜借助荧光染料、荧光蛋白或使用抗体的免疫荧光染色来研究特定的细胞成分。由于荧光剂种类繁多，荧光显微镜可用于检查蛋白质、核酸、聚糖、细胞器和其他细胞结构。
Researchers Insights: Microscopy in Cancer Research
Discover how imaging techniques are driving cancer research forward. In this issue, we present comprehensive multimodal studies using microscopy, as well as new directions in intraoperative cancer…
显微镜中的荧光
荧光显微技术是一种特殊的光学显微镜技术。它利用的是荧光色素在一定波长的光激发下发光的能力。通过抗体染色或荧光蛋白标记，可以用这种荧光色素标记感兴趣的蛋白质。这样就可以确定单分子物种的分布、数量及其在细胞内的定位。此外，还可以进行共定位和相互作用研究，使用可逆结合染料（如 Ca2+ 和 fura-2）观察离子浓度，以及观察细胞的内吞和外吞过程。如今，利用荧光显微镜甚至可以对亚分辨率颗粒进行成像。
High-Pressure Freezing for Organoids: Cryo CLEM & FIB Lift Out
Master cryo EM workflow steps for challenging 3D samples: when to choose HPF vs. plunge freezing, reproducible blotting/ice control, contamination aware transfers, Cryo CLEM 3D targeting in organoids,…
活细胞成像指南
在生命科学各研究领域的广泛应用中，活细胞成像是一种不可或缺的工具，用于观察细胞在尽可能接近活体（即活的、活跃的）状态下的情况。本指南回顾了确保成功进行活细胞成像的各种重要注意事项，并介绍了各种旨在克服常见挑战的高性能解决方案。这些进展使我们能够对细胞生理学和动力学有新的认识。
