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落射荧光显微镜和反射对比显微镜
多年来,荧光显微镜一直仅使用透射光和暗场照明。随着时间的推移,对改进照明的需求不断增长,这导致了落射照明(也称为入射光照明)的发展。经过 40 年的发展和改进,落射照明荧光显微镜已成为生命科学、临床医学诊断和材料科学领域常规实验室工作和研究的实用方法。大部分开发工作由 Ploem 集团和 Leitz 公司(现为 Leica Microsystems)完成。
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Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
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荧光和量子点的基本原理和发展历史
在您的科研生涯的某个时候,都有可能会用到荧光显微镜。这种无处不在的技术改变了显微镜学家对研究对象进行成像、标记和追踪的方式,不论是整个生物体,还是单个蛋白质等等。
通过本文,我们将探讨什么是“荧光”,包括其定义背后的历史和基础物理原理,绿色荧光蛋白(GFP)的发现和应用,并展望量子点等荧光探针不断扩大的应用领域。
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Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
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Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…