![[Translate to chinese:] Co-detection of 10 extracellular matrix proteins and 3 topographical tissue landmarks by multiplex immunostaining within a single high-grade fibrous hotspot from a human hepatocellular carcinoma Single_high-grade_fibrous_hotspot_from_a_human_hepatocellular_carcinoma.jpg](/fileadmin/_processed_/0/8/csm_Single_high-grade_fibrous_hotspot_from_a_human_hepatocellular_carcinoma_c93d3e0744.jpg "[Translate to chinese:] Co-detection of 10 extracellular matrix proteins and 3 topographical tissue landmarks by multiplex immunostaining within a single high-grade fibrous hotspot from a human hepatocellular carcinoma")
了解多重免疫检测的变革潜力
多重成像技术让科学家能够深入研究肿瘤复杂的微环境，揭示肿瘤的独特特征和动态行为，在推动癌症研究方面发挥着至关重要的作用。这项革命性的技术可同时对肿瘤微环境中的各种细胞群及其相互作用进行可视化，从而深入了解肿瘤错综复杂的生态系统。这对于解读肿瘤对治疗干预措施的反应、预测治疗效果以及确定免疫治疗策略的潜在靶点都是不可或缺的。多重成像是一种重要工具，有助于不断探索发现肝细胞癌中的癌症干细胞龛位。
本应用说明强调了多重免疫检测在鉴定和描述肝细胞癌（HCC）内癌症干细胞龛位方面的变革潜力。通过同时检测多种细胞外基质（ECM）蛋白和组织标记，这种创新方法揭示了纤维巢错综复杂的分子组成。这些特殊的肿瘤微环境对了解疾病进展和克服治疗耐药性具有重要意义。
蛋白质组学与多重成像的整合使研究人员有能力揭示肝细胞癌中癌症干细胞龛的复杂细微差别。Cell DIVE 技术的功能与 ClickWell 玻片载体相结合，简化了多重免疫染色过程，揭示了瘤内结构、纤维巢和 ECM 的奥秘。通过 Cell DIVE 的全面抗体验证和迭代染色方法，研究人员可以同时检测多种蛋白质，从而更深入地了解肿瘤微环境。
![[Translate to chinese:] Multiplexed Cell DIVE imaging of Adult Human Alzheimer’s brain tissue section demonstrating expression of markers specific to astrocytes (GFAP, S100B), microglia (TMEM119, IBA1), AD-associated markers (p-Tau217, β-amyloid) and immune cells such as CD11b+, CD163+, CD4+, and HLA-DRA+, clustered around the β-amyloid plaques.](/fileadmin/_processed_/b/b/csm_Alzheimers_brain_tissue_section_showing_astrocytes_microglia_immune_cells_26ab13cde2.jpg "[Translate to chinese:] Multiplexed Cell DIVE imaging of Adult Human Alzheimer’s brain tissue section demonstrating expression of markers specific to astrocytes , microglia , AD-associated markers and immune cells clustered around the β-amyloid plaques")
![[Translate to chinese:] Multiplexed Cell DIVE imaging to characterize the spatial landscape in Human Alzheimer’s Cortical Tissue](/fileadmin/_processed_/7/2/csm_Human_Alzheimers_Cortical_Tissue_Multiplexed_Cell_DIVE_imaging_b053b78344.jpg "[Translate to chinese:] Multiplexed Cell DIVE imaging to characterize the spatial landscape in Human Alzheimer’s Cortical Tissue")
