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How Marine Microorganism Analysis can be Improved with High-pressure Freezing
In this application example we showcase the use of EM-Sample preparation with high pressure freezing, freeze substiturion and ultramicrotomy for marine biology focusing on ultrastructural analysis of…
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![[Translate to chinese:] C. elegans adult hermaphrodite gonades acquired using THUNDER Imager. Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany. [Translate to chinese:] C. elegans adult hermaphrodite gonades acquired using THUNDER Imager. Staining: blue - DAPI (nucleus), green - SP56 (sperm), red - RME-2 (oocyte), magenta - PGL-1 (RNA + protein granules). Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany.](/fileadmin/_processed_/7/c/csm_C_elegans_adult_hermaphrodite_gonades_5c49f08a7f.jpg)
生命科学研究: 哪种显微镜相机适合您?
相机是显微镜系统的重要组成部分,对系统的性能有重大影响。在选择相机时,重要的是不仅要看技术规格,还要考虑您的样品、技术、对比方法以及您希望获得的数据类型。
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考虑采购体视显微镜时的关键因素
体视显微镜通常是实验室或生产现场“主力”。用户需要花费数小时通过目镜来检查、观察、记录或解剖样本。仔细评估哪些相关应用需要用到体视显微镜,是确保长期满意使用的关键所在。决策者们需要确保自己能够完全依照自己的需求来定制仪器。为帮助用户能更好的选择适合自己的体视镜,本文介绍了几个主要考虑的因素。
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![[Translate to chinese:] Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM. [Translate to chinese:] Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM.](/fileadmin/_processed_/2/7/csm_Tilescan_of_brain_organoid_section_364be9e906.jpg)
研究大脑健康的成像类器官模型
小胶质细胞是特化的脑驻留免疫细胞,在大脑发育、平衡和疾病中发挥着至关重要的作用。然而,到目前为止,模拟人脑环境与小胶质细胞之间相互作用的能力还非常有限。
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![[Translate to chinese:] Strain from using a microscope can lead to musculoskeletal pain. [Translate to chinese:] Strain from using a microscope can lead to musculoskeletal pain.](/fileadmin/_processed_/b/1/csm_AdobeStock_musculoskeletal_pain_775fd9b692.jpg)
如何使显微镜工作场所符合人体工学
对于每天使用显微镜工具的人来说,显微镜具有重要影响。显微镜对人体有着很高的要求,需要我们集中注意力,运用肌肉从事大量稳定活动。
在此次采访中,Leica Microsystems的高级产品经理Clinton Smith谈到了如何缓解可能出现的紧张和压力,如何创造符合人体工学的工作场所来帮助显微镜用户舒适地工作,以及如何提高生产率。
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![[Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. [Translate to chinese:] Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.](/fileadmin/_processed_/8/7/csm_Lifetime-based_multiplexing_in_live_cells_using_TauSeparation_c5d58adf13.jpg)
可重复性、协作和新成像技术的力量
在本次网络研讨会上,您将了解到影响显微镜可重复性的因素,有哪些资源和举措可用于改善显微镜教育并提高其严谨性和可重复性以及研究人员、成像科学家和显微镜供应商之间的合作如何推动创新和采用新技术。
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![[Translate to chinese:] Branched organoid growing in collagen where the Nuclei are labeled blue. To detect the mechanosignaling process, the YAP1 is labeled green. [Translate to chinese:] Branched organoid growing in collagen where the Nuclei are labeled blue. To detect the mechanosignaling process, the YAP1 is labeled green.](/fileadmin/_processed_/a/e/csm_Branched_organoid_growing_in_collagen_dc289aa8c6.jpg)
检查癌症类器官的发展进程
德国慕尼黑工业大学的Andreas Bausch实验室研究细胞和生物体中不同结构和功能形成的细胞和生物物理机制。他的团队设计了新的策略、方法和分析工具,以量化微米和纳米等级的发展机制和动态过程。关键研究领域包括干细胞和类器官,从乳腺类器官到胰腺癌类器官,以更好地了解疾病模型。