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Applications of TIRF Microscopy in Life Science Research
The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or…
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Total Internal Reflection Fluorescence (TIRF) Microscopy
Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
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![[Translate to chinese:] Snapshot from a time lapse of a calcium imaging experiment using the ratiometric calcium indicator Fura-2.](/fileadmin/_processed_/5/5/csm_Time_lapse_of_calcium_imaging_experiment_using_the_ratiometric_calcium_indicator_Fura-2_d9ca75ab1a.jpg "[Translate to chinese:] Snapshot from a time lapse of a calcium imaging experiment using the ratiometric calcium indicator Fura-2.")
比例成像
细胞的许多基本功能在很大程度上依赖于离子（例如钙、镁）、电压势和细胞质与周围细胞外空间之间的 pH 值的微妙但动态的平衡。这些平衡的变化会显著改变细胞的行为和功能。因此，实时测量细胞内离子、电压和 pH…
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Optical Contrast Methods
Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
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电生理学和深层组织成像的新标准
神经和肌肉细胞的功能依赖于通过离子通道流动的离子电流。这些离子通道在细胞生理过程中起着关键作用。研究离子通道的一种方法是使用膜片钳技术。这种方法可以详细研究离子通道，并记录不同类型细胞（主要是神经元、肌纤维或胰岛β细胞）的电活动。膜片钳技术由Erwin Neher和Bert…
