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不同组织样品制备方法的RNA质量
本文介绍了样品制备过程和紫外激光显微切片(UV LMD)对小鼠脑组织冷冻切片RNA质量的影响。为在提取RNA时获取良好的结果,从高品质组织开始并在处理前后检查RNA质量至关重要。RNA完整性指数(RIN)以1到10的等级标准来显示样品质量。简言之,RIN数值越高,RNA质量越高。这项研究结果表明,可以利用紫外激光显微切片技术,可以从单个组织细胞中获取品质稳定的RNA。
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荧光显微镜如何为工业应用带来益处
观看这个免费的网络研讨会,了解更多关于荧光显微镜在工业应用中的用途。我们将涵盖一系列调查研究,在这些研究中,荧光对比度为样本属性提供了新的见解,例如纤维、文件、涂料、建筑材料、电子产品和食品的属性。您将看到使用荧光有多么简单,同时还将了解样本制备和潜在局限性。
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冷冻光电联用(Cryo-CLEM)之旅
本文主要介绍Cryo-CLEM技术及其为科学家带来的便益。此外,还特别说明了一些相关文献。
近期在冷冻电子显微镜工作流程领域取得的技术进步,让我们能够获取到细胞蛋白质社会学的3D数据,其分辨率更是达到前所未有的1纳米以下。工作流程中有一个步骤,需要从样品获取目标位置纳米级分辨率的图像,而要得到这样的结果,就需要用到冷冻光学显微镜。这种显微镜如果用于低温电子显微镜工作流程,通常就称为Cryo…
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Fast, High-quality Vitrification with the EM ICE High Pressure Freezer
The EM ICE High Pressure Freezer was developed with a unique freezing principle and uses only a single pressurization and cooling liquid: liquified nitrogen (LN2). This design enables three major…
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使用冷冻共聚焦显微镜定位活性循环核孔复合物
本文介绍了如何利用冷冻光学显微镜,尤其是冷冻共焦显微镜来提高冷冻工作流程的可靠性。评估了EM网格和样品的质量,并分析了目标结构的分布。本文展示了如何将冷冻共焦3D数据投射到SEM图像上,将感兴趣结构可靠地保留在FIB切割的薄片内,以便在冷冻TEM中进行进一步研究。
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Advancing Cell Biology with Cryo-Correlative Microscopy
Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…
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Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own…
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High-pressure freezing: Revealing functional mechanisms of synaptic transmission
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed…
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Workflows and Instrumentation for Cryo-electron Microscopy
Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron…