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Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
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Factors to Consider When Selecting a Research Microscope
An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
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荧光和量子点的基本原理和发展历史
在您的科研生涯的某个时候，都有可能会用到荧光显微镜。这种无处不在的技术改变了显微镜学家对研究对象进行成像、标记和追踪的方式，不论是整个生物体，还是单个蛋白质等等。
通过本文，我们将探讨什么是“荧光”，包括其定义背后的历史和基础物理原理，绿色荧光蛋白（GFP）的发现和应用，并展望量子点等荧光探针不断扩大的应用领域。
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柯勒照明：简史介绍和设置的5个简单步骤
在任何特定光学显微镜调试当中，柯勒照明都是实现最佳成像的最重要且最基本的技术之一。尽管柯勒照明应该视为显微镜设置的常规组成部分，但是许多显微镜学家认为正确的设置过于复杂耗时，因此仍然没有被广泛应用。充分了解显微镜的两个主要部件（光阑和载物台下聚光透镜）在实际操作中的调整后，正确的设置就只需要几分钟的时间。正确对齐的显微镜可以大大改善图像的均匀对比度和照明，从而获得更高的分辨率和观察到更多的细节。在…
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![[Translate to chinese:] Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-illumination using narrow-band blue (490 nm) light. Note the increased image contrast (Ploem, 1967)](/fileadmin/_processed_/c/2/csm_Ploem_Figure_5_Autofluorescence_a_b_fbca553e26.png "[Translate to chinese:] Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-il")
Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
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Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
![[Translate to chinese:] Exchange a fluorescence bulb.](/fileadmin/_processed_/6/2/csm_Exchange_Fluorescence_Bulb_02_df224c13c1.jpg "[Translate to chinese:] Exchange a fluorescence bulb.")
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![[Translate to chinese:] Center a fluorescence bulb.](/fileadmin/_processed_/b/1/csm_Center_Fluorescence_Bulb_02_aa5ac6e7e0.jpg "[Translate to chinese:] Center a fluorescence bulb.")
视频教程： 如何调节荧光光源的灯泡位置
荧光激发的传统光源是带汞灯的荧光灯管。实现明亮均匀激发的前提条件是灯泡在灯罩内正确对中和对齐。
本视频教程介绍了一种简易的方法，用于调节荧光灯管中的汞灯的位置。
![[Translate to chinese:] Jellyfish Aequorea Victoria](/fileadmin/_processed_/7/8/csm_Aequorea3_03_da26f9f6b2.jpg "[Translate to chinese:] Jellyfish Aequorea Victoria")
