Leica DMI6000 B with Adaptive Focus Control DMI6000 加焦平面自动跟踪系统, 用于活细胞研究的全自动显微镜
AFC switched on
COS cells were transfected with a Golgi specific GFP variant. The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of an ice cold salt solution (PBS) was added. Notice that over the complete acquisition time no focus…
AFC switched off
COS cells were transfected with a Golgi specific GFP variant. The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of an ice cold salt solution (PBS) was added. At the time point of adding the ice cold PBS focus drift…