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YFP mouse brain section, 720 µm z stack
Depth color coding.
Sample courtesy of Dr. Mark Lessard, The Jackson Laboratory, Bar Harbor, Maine, USA.
3D animation of a Mouse olfactory bulb
Depth of 1.5mm.
Courtesy of Dr. Günter Giese and Annemarie Scherbarth, MPI Heidelberg, Germany.
See-through brains
Nature Video reveals how Karl Deisseroth and his team created 3D visualizations of mouse brains by using CLARITY and fluorescent labelling.
Thy1-YFP mouse brain treated with CLARITY
Animation generated with LAS X 3D Visualization.
Courtesy of Prof. Karl Deisseroth and Dr. Raju Tomer, Stanford University, Palo Alto, CA, USA.
Mouse lymph node with GFP-labelled macrophages
SHG signal from collagen (grey).
Zebrafish (gastrulation) RNA injection for Neptune fluorescent protein cytoplasmic staining
THG (in blue); 20x1NA; InSight at 1140 nm.
Courtesy of BioEmergences USR3695 (IBiSA FBI), Gif sur Yvettes, France.
Zebrafish embryo development over 21h
4D time lapse, 420 stacks, 3 channel overlay: RNA injection H2B (blue), mCherry (red), EGFPras (green).
Courtesy of BioEmergences USR3695 (IBiSA FBI), Gif sur Yvettes, France.
SHG signal and a parasympathetic neuronal marker in cleared pancreas
Tyrosine Hydroxylase positive neurons (red), SHG at 1080nm (green), Dapi (blue).
Courtesy of Dr. Rafael Drigo, Per-Olof Berggren Laboratory, Lee Kong Chian School of Medicine (LKCSoM), Singapore.
Multicolor image of a Zebrafish embryo
Lateral line primordium labeled with GFP (Green), neurons with DsRed (red), nucleus with BFP (blue). SHG signal from muscles (grey).
Courtesy of Dr. Lionel Newton, EMBL Heidelberg, Germany.
Calcium movement in GCaMP3 mouse after podocyte injury
Calcium movement after podocyte injury (bottom right) in a glomerulus of a GCaMP3 mouse. Vasculature labeling by Texas Red.
Courtesy of Dr. Matthias Hackl, University Clinic Cologne, Germany.
Tunicate (Phallusia mammillata) development over 6,5 h
Staining of the nuclei through RNA injection (H2B-eGFP, 980nm); membrane through bathing in FM4-64 (1030nm).
Courtesy of BioEmergences USR3695 (IBiSA FBI), Gif sur Yvettes, France.
Simultaneous live rotation and IR excitation
Mouse testis section expressing dTomato (OPO).
Sample courtesy of Dr. Mark Lessard, The Jackson Laboratory, Bar Harbor, Maine, USA.
Live imaging of Microglias in a live, awake mouse
Movement of Microglias in a live awake mouse, 4D animation of 217 slices and 180 sec.
Courtesy of Dr. Masahiro Fukuda, Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.
Astyanax mexicanus surface fish development over 30 h
RNA injection for staining by farnesylated GFP (membranes, 980nm) and H2B-mCherry (nuclei, 1030nm).
Courtesy of BioEmergences USR3695 (IBiSA FBI), Dr. Sylvie Rétaux, Dr. Hélène Hinaux and Dr. Gaëlle Recher, CNRS, Gif sur Yvette, France.
In vivo Thy1-EYFP mouse, z stack
Adult Thy1-EYFP H line mouse, in vivo (cranial window), 800 µm z stack.
Courtesy of Dr. Masahiro Fukuda, Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.
Zebrafish Development for 17 hours
Expression of cytoplasmic GFP in the notochord and prechordal plate, 980nm. Nuclei stain through RNA injection H2B-mCherry, 1030 nm.
Courtesy of BioEmergences USR3695 (IBiSA FBI), Gif sur Yvettes, France.
Adult Thy1-EYFP H line mouse, in vivo (cranial window)
Excitatory pyramidal neurons in Layer 5 partly express EYFP.
Animation created with LAS AF 3 3D Visualization package, Leica Microsystems.
Dr. Masahiro Fukuda, Prof. Haruo Kasai, Center for Disease Biology and Integrative Medicine, Faculty of…
